抗体 > 标签内参抗体 > 6*His Monoclonal Antibody
  • 产品类型:
    Tag/Control Antibodies
  • 产品名称:

    6*His Monoclonal Antibody

  • 货号:
    CSB-MA000011M0m
  • 规格:
    ¥1,200
  • 免疫原:
    HHHHHH(6x His) synthetic peptide conjugate to KLH
  • 宿主:
    Mouse
  • 应用范围:
    ELISA, WB; Recommended dilution: WB:1:500-1:5000
  • 背景:
    A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    IgG2a
  • 纯化方式:
    >95%,Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

    All lanes :Mouse Anti-6*his monoclonal antibody at 1ug/ml
    Western blot analysis of 6*his Epitope Tag was performde by loading various amounts of lysates containing different HIS tagged
    Recombinant proteins per well onto a 15% Tris-HCL polyacrylamide gel.
    Line 1: FABP3 insect cell lysate
    Line 2: TK1 insect cell lysate
    Line 3: cagA insect cell lysate
    Line 4: NGAL insect cell lysate
    Line 5: IGFBP1 293T cell lysate
    Line 6: TRY2 293T cell lysate

    All lanes : Recombinant DST Protein at 30ng/ml
    Line 1: Mouse Anti-his monoclonal antibody at 1:64000
    Line 2: Mouse Anti-his monoclonal antibody at 1:32000
    Line 3: Mouse Anti-his monoclonal antibody at 1:16000
    Line 4: Mouse Anti-his monoclonal antibody at 1:8000
    Line 5: Mouse Anti-his monoclonal antibody at 1:4000
    Observed band size : 35kd
    Predicted band size : 35kd

  • 说明书:

    注:产品说明书可能有改进,请直接与CUSABIO产品专员联系,索取最新版说明书!

  • 别名:
    6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
  • 引用文献:
    1. Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer\\\'s disease brain , Dominguez G.et al,Acta Neuropathol Commun,Apr 03