抗体 > 多克隆抗体 > CD33 Antibody
  • 产品类型:
    Polyclonal Antibody
  • 产品名称:

    CD33 Antibody

  • 货号:
    CSB-PA004925LA01HU
  • 规格:
    ¥1200
  • 别名:
    Myeloid cell surface antigen CD33 (Sialic acid-binding Ig-like lectin 3) (Siglec-3) (gp67) (CD antigen CD33), CD33, SIGLEC3
  • 种属:
    Human
  • 免疫原:
    Recombinant Human Myeloid cell surface antigen CD33 protein (18-259AA)
  • 宿主:
    Rabbit
  • 种属反应性:
    Human
  • 应用范围:
    ELISA, WB, IHC; Recommended dilution: WB:1:500-5000, IHC:1:200-1:500
  • 背景:
    Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

    Western Blot
    Positive WB detected in: HL60 whole cell lysate
    All lanes: CD33 antibody at 3ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 40, 34, 26 kDa
    Observed band size: 40 kDa

    IHC image of CSB-PA004925LA01HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • 说明书:
  • Accession No.:
    P20138
  • 研究领域:
    Immunology
  • 引用文献:
    1. Shape-Shifted Red Blood Cells: A Novel Red Blood Cell Stage? , Chico V.et al,Cells,19 April