试剂盒 > 酶联免疫试剂盒 > 猪髓磷脂碱性蛋白(MBP)ELISA Kit
    • ELISA Kit
    • 猪髓磷脂碱性蛋白(MBP)ELISA Kit
    • The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called Golli-MBP ) that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.[provided by RefSeq]
    • UniProtP81558
    • HGNC3350
    • RGD2553
    • MGI95393
    • OMIM172430
    • CUSABIOCSB-E07964p
  • 产品类型:
    ELISA Kit
  • 产品名称:

    猪髓磷脂碱性蛋白(MBP)ELISA Kit

  • 英文名称:

    Pig myelin basic protein,MBP ELISA Kit

  • 货号:
    CSB-E07964p
  • 别名:
    MGC99675, Golli-mbp|OTTHUMP00000174383
  • 规格:
    96T
  • 中文价格:
    4200
  • 种属:
    Pig
  • 其他种属:
  • 待测物名称:
    myelin basic protein
  • 缩写:
    MBP
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.094 2.074 2.084 1.915
    101.408 1.435 1.422 1.253
    50.880 0.899 0.890 0.721
    2.50.611 0.636 0.624 0.455
    1.250.425 0.457 0.441 0.272
    0.6250.339 0.361 0.350 0.181
    0.3120.284 0.298 0.291 0.122
    00.168 0.170 0.169
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %81-89
    1:2Average %96
    Range %93-99
    1:4Average %94
    Range %90-99
    1:8Average %87
    Range %82-90
  • 回收率:
    The recovery of pig MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9592-99
    EDTA plasma (n=4)104100-108
  • 研究领域:
    Neuroscience
  • 引用文献:
    1. Can recognition of spinal ischemia be improved? Application of motor-evoked potentials, serum markers and breath gas analysis in an acutely instrumented pig model , Pschel A.et al,Ann Vasc Surg,Mar 05
    2. Can Recognition of Spinal Ischemia Be Improved? Application of Motor-Evoked Potentials, Serum Markers, and Breath Gas Analysis in an Acutely Instrumented Pig Model , Anja Puschel.et al,ANN VASC SURG,April