试剂盒 > 酶联免疫试剂盒 > 小鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA Kit
    • ELISA Kit
    • 小鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA Kit
    • This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.[provided by RefSeq]
    • UniProtP10148
    • CUSABIOCSB-E07430m
  • 产品类型:
    ELISA Kit
  • 产品名称:

    小鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)ELISA Kit

  • 英文名称:

    Mouse monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP-1/MCAF ELISA kit

  • 货号:
    CSB-E07430m
  • 别名:
    GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte secretory protein JE|small inducible cytokine
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Mouse
  • 待测物名称:
    chemokine (C-C motif) ligand 2
  • 缩写:
    CCL2
  • 蛋白功能1:
    Immunity
  • 蛋白功能2:
    chemokine
  • 蛋白功能3:
    Chemotaxis
  • 检测范围:
    78 pg/mL-5000 pg/mL
  • 灵敏度:
    19.5 pg/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50001.946 1.864 1.905 1.806
    25001.294 1.414 1.354 1.255
    12500.861 0.843 0.852 0.753
    6250.555 0.546 0.551 0.452
    3120.347 0.356 0.352 0.253
    1560.267 0.258 0.263 0.164
    780.171 0.179 0.175 0.076
    00.099 0.098 0.099  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %88
    Range %82-94
    1:2Average %95
    Range %91-98
    1:4Average %102
    Range %96-108
    1:8Average %95
    Range %89-98
  • 回收率:
    The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8882-94
    EDTA plasma (n=4)9792-104
  • 研究领域:
    Immunology
  • 引用文献:
    1. The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro , Zong S.et al,Cell Physiol Biochem.,2016,38(3):1100-10.
    2. p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes. , Yu X.et al,Gene.,589(1): 12-19.
    3. n-Hexane Insoluble Fraction of Plantago lanceolata Exerts Anti-Inflammatory Activity in Mice by Inhibiting Cyclooxygenase-2 and Reducing Chemokines Levels , Nanang Fakhrudin.et al,Sci Pharm., 13 March 2017, 85(1), 12;
    更多文献